海兰鸡内源性白血病病毒位点序列鉴定与分析

doi:10.11843/j.issn.0366-6964.2015.11.018

Abstract

Abstract:

To study the integration site of endogenous avian leukosis virus（ALV） elements in the genome of Hy-Line Variety Brown，a non-joined PCR amplicon（named HL-E1） was obtained using a B family DNA polymerase from hypothermophilic archaea Thermococcus sp.4557（TXW-PolB）.HL-E1 contains three essential genes including gag，pol，and env and the 3’-LTRs.And it lacks 93，1 960 and 805 base pairs in the gag，pol and env genes，respectively.A 4 663 bp functional mutant of ALV integrated in the Hy-Line Variety Brown genome.The conserved env（highly similar with endogenous avian leucosis virus） and LTR genes have identities of 96.8% and 97.4% to ev1 site.This indicates a newly identified endogenous ALV sequence integrated in an unknown site in the chicken genome.

CLC Number:

S852.659.3

CHEN Zi-meng，DONG Xuan，SU Shuai，CUI Ning，LI Zhuo，CUI Zhi-zhong. Identification and Sequence Analysis of Endogenous Avian Leukosis Virus Element in Genome of Hy-Line Brown Layers[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(11): 2050-2055.

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References

［1］COFFIN J M.Structure and Classification of Retroviruses［M］.New York：Plenum Press，1992，1：19-49.
［2］王鑫，赵鹏，崔治中.我国地方品种鸡分离到的一个禽白血病病毒新亚群的鉴定［J］.病毒学报，2012，28（6）：609-614.
WANG X，ZHAO P，CUI Z Z.Identification of a new subgroup of avian leukosis virus isolated from Chinese indigenous chicken breeds［J］. Chinese Journal of Virology， 2012，28（6）：609-614.（in Chinese）
［3］SAIF Y M，BARNES H J，GLISSON J R，et al.Disease of poultry［M］.Iowa State University Press，2003：64-78.
［4］SPENCER J L，GILKA F，GAVORA J S，et al.Distribution of lymphoid leukosis virus and p27 group-specific antigen in tissues from laying hens［J］. Avian Dis，1984，28（2）：358-373.
［5］CRITTENDEN L B，SMITH E J.A comparision of test materials for differentiating avian leukosis virus group-specific antigens of exogenous and endogenous origin［J］.Avian Dis，1984，28（4）：1057-1070.
［6］ASTRIN S M.Endogenous viral genes of the White Leghorn chicken：common site of residence and sites associated with specific phenotypes of viral gene expression［J］.Proc Natl Acad Sci U S A，1978，75（12）：5941-5945.
［7］DUNWIDDIE C T，RESNICK R，BOYCE-JACINO M，et al.Molecular cloning and characterization of gag-，pol-，and env-related gene sequences in the ev-chicken［J］. J Virol，1986，59（3）：669-675.
［8］SACCO M A，FLANNERY D M，HOWES K，et al.Avian endogenous retrovirus EAV-HP shares regions of identity with avian leukosis virus subgroup J and the avian retrotransposon ART-CH［J］. J Virol，2000，74（3）：1296-1306.
［9］STUMPH W E，HODGSON C P，TSAI M J，et al.Genomic structure and possible retroviral origin of the chicken CR1 repetitive DNA sequence family［J］.Proc Natl Acad Sci U S A，1984，81（21）：6667-6671.
［10］HUDA A，POLAVARAPU N，JORDAN I K，et al.Endogenous retroviruses of the chicken genome［J］.Biol Direct，2008，3：9.
［11］BACON L D，FADLY A M，CRITTENDEN L B.Absence of influence on immune competence by the sex-linked gene（K） determining slow feathering in White Leghorn chickens［J］.Avian Dis，1986，30（4）：751-760.
［12］BACON L D，SMITH E，CRITTENDEN L B，et al.Association of the slow feathering（K） and an endogenous viral（ev21） gene on the Z chromosome of chickens［J］.Poult Sci，1988，67（2）：191-197.
［13］CRITTENDEN L B.Retroviral elements in the genome of the chicken：implications for poultry genetics and breeding［J］.Crit Rev Poult Biol，1991，3（2）：73-109.
［14］SMITH E J，CRITTENDEN L B.Endogenous viral genes in a slow-feathering line of white leghorn chickens［J］.Avian Pathol，1986，15（3）：395-406.
［15］BENKEL B F.Locus-specific diagnostic tests for endogenous avian leukosis-type viral loci in chickens［J］.Poult Sci，1998，77（7）：1027-1035.
［16］SMITH A，BENKEL B F.Novel avian leukosis virus-related endogenous proviruses from layer chickens：Characterization and development of locus-specific assays［J］.Poult Sci，2009，88（8）：1580-1585.
［17］YANG J，YU Y，YAO J，et al.Molecular identification of avian leukosis virus subgroup E loci and tumor virus B locus in Chinese indigenous chickens［J］.Poult Sci，2011，90（4）：759-765.
［18］RUTHERFORD K，BENKEL B F.Characterization of insertion sites and development of locus-specific assays for three broiler-derived subgroup E avian leukosis virus proviruses［J］.Avian Pathol，2013，42（4）：373-378.
［19］International Chicken Genome Sequenicing Consortium.Sequence and comparative analysis of the chicken genome provide unique perspectives on vertebrate evolution［J］.Nature，2004，432（7018）：695-716.
［20］徐海鹏，孟凡峰，董宣，等.种蛋中内源性禽白血病病毒的检测和鉴定［J］.畜牧兽医学报，2014，45（8）：1317-1323.
XU H P，MENG F F，DONG X，et al.Detection and identification of an endogenous subgroup E avian lekosis virus in a chicken breeder embroy［J］.Acta Veterinaria et Zootechnica Sinica，2014，45（8）：1317-1323.（in Chinese）
［21］刘超男，高玉龙，高宏雷，等.J 亚群与 E 亚群禽白血病自然重组病毒的全基因组序列分析［J］.中国预防兽医学报，2009，31（12）：978-981.
LIU C N，GAO Y L，GAO H L，et al.Molecular characterization of a natural recombinant subgroup J avian leucosis virus with subgroup E partial env gene［J］.Chinese Journal of Preventive Veterinary Medicine，2009，31（12）：978-981.（in Chinese）
［22］孔义波，张兴晓，姜世金，等.SPF 鸡胚中内源性白血病病毒全基因序列鉴定与分析［J］.病毒学报，2008，24（1）：53-58.
KONG Y B，ZHANG X X，JIANG S J，et al.Sequence analysis for the complete provial genome of endogenous avian leukosis virus strian SD0501［J］.Chinese Journal of Virology，2008，24（1）：53-58.（in Chinese）
［23］CUI Z，SUN S，WANG J.Reduced serologic response to Newcastle disease virus in broiler chickens exposed to a Chinese field strain of subgroup J avian leukosis virus［J］. Avian Dis，2006，50（2）：191-195.
［24］CUI N，SU S，CHEN Z，et al.Genomic sequence analysis and biological characteristics of a rescued clone of avian leukosis virus strain JS11C1，isolated from indigenous chickens［J］.J Gen Virol，2014，95（Pt 11）：2512-2522.
［25］WANG X，GAO Z，XU X，et al.Complete genome sequence of Thermococcus sp.strain 4557，a hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent area［J］.J Bacteriol，2011，193（19）：5544-5545.
［26］JOHNSON J A，HENEINE W.Characterization of endogenous avian leukosis viruses in chicken embryonic fibroblast substrates used in production of measles and mumps vaccines［J］.J Virol，2001，75（8）：3605-3612.

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Identification and Sequence Analysis of Endogenous Avian Leukosis Virus Element in Genome of Hy-Line Brown Layers

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